slides
Unicycler assembly of SARS-CoV-2 genome with preprocessing to remove human genome reads
Abstract
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Questions this will address
- How can a genome of interest be assembled against a background of contaminating reads from other genomes?
- How can sequencing data used to obtain an assembled genome?
Learning Objectives
- Know basic characteristics of SARS-CoV-2
- Understand Nanopore and Illumina technologies
- Detect and remove human reads from SARS-CoV-2 sequencing reads
- Know main de novo genome assembly algorithms
- Perform a hybrid de novo genome assembly
Licence: Creative Commons Attribution 4.0 International
Keywords: Assembly, covid19
Target audience: Students
Resource type: slides
Version: 6
Status: Active
Prerequisites:
- Introduction to Galaxy Analyses
- Quality Control
Learning objectives:
- Know basic characteristics of SARS-CoV-2
- Understand Nanopore and Illumina technologies
- Detect and remove human reads from SARS-CoV-2 sequencing reads
- Know main de novo genome assembly algorithms
- Perform a hybrid de novo genome assembly
Date modified: 2023-04-11
Date published: 2021-01-07
Contributors: Cristóbal Gallardo
Scientific topics: Sequence assembly
Activity log