slides

Unicycler assembly of SARS-CoV-2 genome with preprocessing to remove human genome reads

Abstract

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Questions this will address

  • How can a genome of interest be assembled against a background of contaminating reads from other genomes?
  • How can sequencing data used to obtain an assembled genome?

Learning Objectives

  • Know basic characteristics of SARS-CoV-2
  • Understand Nanopore and Illumina technologies
  • Detect and remove human reads from SARS-CoV-2 sequencing reads
  • Know main de novo genome assembly algorithms
  • Perform a hybrid de novo genome assembly

Licence: Creative Commons Attribution 4.0 International

Keywords: Assembly, covid19

Target audience: Students

Resource type: slides

Version: 6

Status: Active

Prerequisites:

  • Introduction to Galaxy Analyses
  • Quality Control

Learning objectives:

  • Know basic characteristics of SARS-CoV-2
  • Understand Nanopore and Illumina technologies
  • Detect and remove human reads from SARS-CoV-2 sequencing reads
  • Know main de novo genome assembly algorithms
  • Perform a hybrid de novo genome assembly

Date modified: 2023-04-11

Date published: 2021-01-07

Authors: Cristóbal Gallardo

Contributors: Cristóbal Gallardo

Scientific topics: Sequence assembly


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