e-learning

Understanding Barcodes

Abstract

Barcodes are small oligonucleotides that are inserted into the captured sequence at a specific point, and provide two pieces of information about the sequence:

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • What are barcodes?
  • What is their purpose?
  • How are barcodes formatted?

Learning Objectives

  • Demultiplex FASTQ data via UMI-tools
  • Understand and validate the extraction of barcodes

Licence: Creative Commons Attribution 4.0 International

Keywords: Single Cell

Target audience: Students

Resource type: e-learning

Version: 7

Status: Active

Prerequisites:

Introduction to Galaxy Analyses

Learning objectives:

  • Demultiplex FASTQ data via UMI-tools
  • Understand and validate the extraction of barcodes

Date modified: 2024-06-14

Date published: 2019-02-20

Authors: Mehmet Tekman

Contributors: Mehmet Tekman

Scientific topics: Transcriptomics

External resources:

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