e-learning

Mapping

Abstract

Sequencing produces a collection of sequences without genomic context. We do not know to which part of the genome the sequences correspond to. Mapping the reads of an experiment to a reference genome is a key step in modern genomic data analysis. With the mapping the reads are assigned to a specific location in the genome and insights like the expression level of genes can be gained.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • What is mapping?
  • What two things are crucial for a correct mapping?
  • What is BAM?

Learning Objectives

  • Run a tool to map reads to a reference genome
  • Explain what is a BAM file and what it contains
  • Use genome browser to understand your data

Licence: Creative Commons Attribution 4.0 International

Keywords: Sequence analysis

Target audience: Students

Resource type: e-learning

Version: 41

Status: Active

Prerequisites:

  • Introduction to Galaxy Analyses
  • Quality Control

Learning objectives:

  • Run a tool to map reads to a reference genome
  • Explain what is a BAM file and what it contains
  • Use genome browser to understand your data

Date modified: 2024-09-22

Date published: 2016-10-04

Authors: Bérénice Batut, Helena Rasche, Joachim Wolff

Contributors: Bérénice Batut, Helena Rasche, Joachim Wolff

Scientific topics: Sequence analysis, Mapping


Activity log