e-learning
Essential genes detection with Transposon insertion sequencing
Abstract
In microbiology, identifying links between genotype and phenotype is key to understand bacteria growth and virulence mechanisms, and to identify targets for drugs and vaccines. These analysis are limited by the lack of bacterial genome annotations (e.g. 30% of genes for S. pneumoniae are of unknown function) and by the fact that genotypes often arose from complex composant interactions.
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- What is Transposon insertion Sequencing?
- How to get TA sites Coverage ?
- How to predict essential genes ?
Learning Objectives
- Understand the read structure of TnSeq analyses
- Predict Essential genes with Transit
- Compare gene essentiality in control sample and an different experimental condition
Licence: Creative Commons Attribution 4.0 International
Keywords: Genome Annotation, bacteria, essential genes, microgalaxy, tnseq
Target audience: Students
Resource type: e-learning
Version: 12
Status: Active
Prerequisites:
Introduction to Galaxy Analyses
Learning objectives:
- Understand the read structure of TnSeq analyses
- Predict Essential genes with Transit
- Compare gene essentiality in control sample and an different experimental condition
Date modified: 2024-03-18
Date published: 2019-07-02
Contributors: Bérénice Batut, Delphine Lariviere
Scientific topics: Genomics, Microbiology, Sequence analysis, Mobile genetic elements
Activity log
