e-learning

De novo transcriptome assembly, annotation, and differential expression analysis

Abstract

As a result of the development of novel sequencing technologies, the years between 2008 and 2012 saw a large drop in the cost of sequencing. Per megabase and genome, the cost dropped to 1/100,000th and 1/10,000th of the price, respectively. Prior to this, only transcriptomes of organisms that were of broad interest and utility to scientific research were sequenced; however, these developed in 2010s high-throughput sequencing (also called next-generation sequencing) technologies are both cost- and labor- effective, and the range of organisms studied via these methods is expanding.

About This Material

This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.

Questions this will address

  • Which biological questions are addressed by the tutorial?
  • Which bioinformatics techniques are important to know for this type of data?

Learning Objectives

  • The learning objectives are the goals of the tutorial
  • They will be informed by your audience and will communicate to them and to yourself what you should focus on during the course
  • They are single sentences describing what a learner should be able to do once they have completed the tutorial
  • You can use Bloom's Taxonomy to write effective learning objectives

Licence: Creative Commons Attribution 4.0 International

Keywords: Transcriptomics

Target audience: Students

Resource type: e-learning

Version: 8

Status: Draft

Prerequisites:

  • Introduction to Galaxy Analyses
  • Mapping
  • Quality Control

Learning objectives:

  • The learning objectives are the goals of the tutorial
  • They will be informed by your audience and will communicate to them and to yourself what you should focus on during the course
  • They are single sentences describing what a learner should be able to do once they have completed the tutorial
  • You can use Bloom's Taxonomy to write effective learning objectives

Date modified: 2023-11-09

Date published: 2020-02-13

Authors: Anthony Bretaudeau, Erwan Corre, Gildas Le Corguillé, Xi Liu

Contributors: Anthony Bretaudeau, Erwan Corre, Gildas Le Corguillé, Xi Liu

Scientific topics: Transcriptomics


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