e-learning
Annotating a protein list identified by LC-MS/MS experiments
Abstract
ProteoRE Galaxy instance provides necessary tools to execute a whole annotation pipeline of a protein list identified by LC-MS/MS experiments. This activity introduces these tools and guides you through a simple pipeline using some example datasets based on the study entitled "Proteomic characterization of human exhaled breath condensate".
About This Material
This is a Hands-on Tutorial from the GTN which is usable either for individual self-study, or as a teaching material in a classroom.
Questions this will address
- How to filter out technical contaminants?
- How to check for tissue-specificity?
- How to perform enrichment analysis?
- How to map your protein list to pathways (Reactome)?
- How to compare your proteome with other studies?
Learning Objectives
- Execute a complete annotation pipeline of a protein list identified by LC-MS/MS experiments
Licence: Creative Commons Attribution 4.0 International
Keywords: DDA, Proteomics, human
Target audience: Students
Resource type: e-learning
Version: 11
Status: Active
Prerequisites:
Introduction to Galaxy Analyses
Learning objectives:
- Execute a complete annotation pipeline of a protein list identified by LC-MS/MS experiments
Date modified: 2024-06-14
Date published: 2019-09-12
Contributors: David Christiany, Florence Combes, Valentin Loux, Yves Vandenbrouck
Scientific topics: Proteomics
Activity log