Educators:
Prof. Dr. Stefan Janssen, Anna Rehm (BiGi)

Date:
Oct 8th-10th 2024

Location:
Justus Liebig University
Heinrich-Buff-Ring 58
35392 Giessen
Room 0024a

Contents:
The microbiome is the ecological community of commensal, symbiotic and pathogenic microorganisms found in and on all multicellular organisms from plants to animals. It has been found to be crucial for immunologic, hormonal and metabolic homeostasis of its host. In this workshop, we aim to not only introduce you to the all the necessary basic methods to analyse a microbiome experiment, like processing your raw data, statistical testing and quality control. Additionally, we want to introduce you to Qiita, a microbial study management platform used to manage and compute studies with microbiome data. This framework establishes an easily accessible way for non-computational scientists to analyse their own data with the option of using pre-defined workflows or building their own. Afterwards, we will explore the microbiome analysis pipeline QIIME2 to delve further into our data sets and to enable you to be able to take your first steps towards your own analysis.

Learning goals:
• To understand the principles and applications of 16S amplicon sequencing for microbiome research
• To learn how to design, perform, and troubleshoot 16S amplicon experiments, including sample collection, DNA extraction, PCR amplification, library preparation, and sequencing
• To acquire the skills and tools to process, analyze, and interpret 16S amplicon data using various bioinformatics platforms
• To explore the diversity, structure, and function of microbial communities across different environments, hosts, and conditions using 16S amplicon data
• To critically evaluate the quality, validity, and limitations of 16S amplicon data and result

Prerequisites:
None

Keywords:
16S Amplicon, Microbial Omics

Tools:
Qiita, QIIME2