Workshops and courses

ELIXIR node event NEOF - Bacterial 16S metabarcoding

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Date: 11 - 13 February 2025

Timezone: London

Language of instruction: English

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Sequencing of the 16S rRNA gene is a well established method of determining the bacterial taxonomic composition of microbiomes. This has
been used for human and animal body sites, soil, sewage, clouds, deserts, permafrost and many other environments. This course will give
you the ability to describe the advantages and disadvantages of 16S rRNA sequencing, and analyse 16S rRNA datasets with the QIIME2
bioinformatics platform.

Places are restricted to UK-based researchers and only 30 places are available so please only register if you intend on attending.

The workshop will be held over two days on Tuesday 11th and Thursday 13th February 2025, 10am-4pm, with registration opening 9.30am on Tuesday.

The deadline to register is Monday 27/1/25 noon, 2 weeks before the course. You will hear later that week whether you have been invited and will need to confirm your attendance.

Register to NEOF mailing list to hear first about future NEOF free bioinformatics courses: https://neof.org.uk/news/

City: Online

Learning objectives:

At the end of the course learners will be able to:

  • Utilise and understand the use of QIIME2 with 16S rRNA data.
  • Import sequencing data and metadata into QIIME2 artifacts.
  • Cluster and denoise 16S sequences with DADA2.
  • Assign taxonomies to ASVs and produce a phylogenetic tree.
  • Evaluate and normalise sequencing depth across their samples.
  • Produce alpha and beta diversity metrics and statistics.
  • Carry out differential abundance analysis.

Organizer: NEOF

Event types:

  • Workshops and courses

Sponsors: NERC

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