The goal of this course is to provide an overview of in-field, real-time nanopore sequencing using the Oxford Nanopore Technologies (ONT) platform. This course will cover experimental considerations, sample collection and preparation theory, plus data analysis and visualisation. Hands-on opportunities for data analysis of metagenomic data will cover the use of EI’s MARTi software and will be provided via virtual machines. You will also have the opportunity to discuss and apply lessons to your own projects with EI’s experts and a representative from Oxford Nanopore Technologies during an optional Q&A session.

What will I learn?

Experimental considerations for nanopore sequencing
Advantages and disadvantages of different extraction protocols
Familiarity with different DNA QC and library construction methods
Familiarity with nanopore sequencing outputs
Common analysis tools for alignment, assembly and classification of nanopore data
Capabilities of nanopore’s epi2me cloud-based data analysis platform
Effective use of MARTi software for real-time data analysis for species identification and quantification