Workshops and courses
Learning Pathway Clinical metaproteomics workflows within Galaxy
Date: No date given
This learning path aims to teach you the basics of how to perform metaproteomics analysis of the clinical data within the Galaxy platform. You will learn how to use Galaxy for analysis and will be guided through the most common first steps of any metaproteomics database generation to searching the database, verifying the proteins/peptides, and data analysis.
Keywords: beginner
Learning objectives:
- Analyze significant proteins
- Downloading databases related to 16SrRNA data
- Extract confident peptides and proteins
- For better identification results, combine host and microbial proteins.
- Generate a microbial peptide panel for verification
- Look at the taxonomic distribution of the quantified peptides
- Perform Database searching using two algorithms
- Perform group comparison analysis.
- Perform quantitation using MaxQuant and extract microbial and human proteins and peptides.
- Reduced database provides better FDR stats.
- Verification of peptides helps in confirming the presence of the peptides in our samplle
Event types:
- Workshops and courses
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