Whole genome sequencing (WGS) has been revolutionised by the development of long-read sequencing technologies in the last few years. Driven in no small part by Oxford Nanopore technologies (https://nanoporetech.com/) and Pacific Biosciences, HiFi (https://www.pacb.com/technology/hifi-sequencing/) we now have the ability to sequence long (mb+) single-molecule DNA fragments. Although these developments are expected to alleviate numerous computational challenges surrounding genome analysis they also bring some interesting bioinformatics challenges to which we have to adapt in order to get the most from this powerful technology.