This course if aimed for those who seek to deepen their biostatistical and machine learning skills. Building on the Introduction to Biostatistics and Machine Learning course, this course expands on common life science data analysis methods, including dimensionality reduction techniques beyond PCA, mixed-effects models for analysis of repeated measures, and survival analysis. We will also dive deeper into machine learning, covering more classification algorithms, ensemble techniques, optimization strategies and PLS methods for single and multi-omics data analysis.