The VIB Proteomics Core offers contemporary mass spectrometry (MS)-based proteomics services to VIB scientists. During this course we provide lectures on the basic principles and theory behind MS-based proteomics.The goal is to provide participants with insight on how to design their experiments and how to select the right sample preparation procedure and labeling strategy.During the hands-on lab sessions, participants will be trained in sample preparation including protein extraction, digestion and peptide clean-up for LC-MS/MS analysis.We also include a data analysis demonstration session, covering critical steps in proteomics data analysis including protein identification, quantification, statistical analysis and data visualization.